Faculty Advisor

Heilman, Destin

Faculty Advisor

Politz, Samuel M.

Center

University of Massachusetts Medical Center

Abstract

The goal of this project was to develop high-throughput protein and peptide loading of beta glucan particles (GPs) for vaccine development in 96-well plates. After establishing ideal loading conditions to be similar to those of standard GP loading, peptide binding using N-succinimidyl S-acetylthioacetate (SATA) and a new binding method using 2,2'-dithiolethyl bis(4-azido-2,3,5,6-tetrafluorobenzoate) (DAT) showed that the DAT GPs bound peptide more efficiently than the SATA GPs. GP-mediated peptide delivery to OT-II T-cells showed that DAT-derivatized GPs stimulated a stronger immune response than linear PEI/SATA-derivatized GPs. Immunological testing for Ova protein-loaded GPs versus IgG- and lysozyme-loaded GPs showed that both Ova protein- and IgG-loaded GPs stimulated a T-cell response.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2014

Major

Biology and Biotechnology

Major

Chemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

Share

COinS