Dempski, Robert E.
The goal of this project was to express, purify, and characterize the M3M4 intracellular region of the ZIP4 protein. This region has a series of histidine residues, which have a high zinc binding affinity. The zinc binding affinity and induced conformational change of the M3M4 region was measured using isothermal titration calorimetry and circular dichroism. The result of this project was the standardization and optimization of the protein purification protocol and zinc binding assays, as well as confirmation of previous data.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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Chemistry and Biochemistry