Faculty Advisor

Dempski, Robert E.

Abstract

The goal of this project was to express, purify, and characterize the M3M4 intracellular region of the ZIP4 protein. This region has a series of histidine residues, which have a high zinc binding affinity. The zinc binding affinity and induced conformational change of the M3M4 region was measured using isothermal titration calorimetry and circular dichroism. The result of this project was the standardization and optimization of the protein purification protocol and zinc binding assays, as well as confirmation of previous data.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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