Faculty Advisor

Lambert, Christopher R.

Faculty Advisor

McGimpsey, W. Grant

Abstract

The goal of this project was to produce ethanol using a magnetically stabilized, continuous, bioreactor. This was achieved by the entrapment of Saccharomyces Cerevisiae yeast in calcium alginate beads, doped with magnetite. The bioreactor was constructed in-house and consisted of a flow through chamber in which the beads were stabilized with an array of magnets. Ethanol production was monitored over a period of up to 8 days using gas chromatography analysis of headspace vapor. Immobilization of yeast cells allowed for the optimization of ethanol production. With a working volume of 250mL, a 13% ethanol concentration was achieved within 72 hours. The system may be used for biofuel production ideally using waste as feedstock and appropriate selection of the entrapped organism.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Chemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

BEI

Advisor Department

Chemistry and Biochemistry

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