Lambert, Christopher R.
McGimpsey, W. Grant
The goal of this project was to produce ethanol using a magnetically stabilized, continuous, bioreactor. This was achieved by the entrapment of Saccharomyces Cerevisiae yeast in calcium alginate beads, doped with magnetite. The bioreactor was constructed in-house and consisted of a flow through chamber in which the beads were stabilized with an array of magnets. Ethanol production was monitored over a period of up to 8 days using gas chromatography analysis of headspace vapor. Immobilization of yeast cells allowed for the optimization of ethanol production. With a working volume of 250mL, a 13% ethanol concentration was achieved within 72 hours. The system may be used for biofuel production ideally using waste as feedstock and appropriate selection of the entrapped organism.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry
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