Faculty Advisor

Adams, David S.

Abstract

The goal of this project was to study the toxicity and anti-tumor effects of chemotherapy drugs Doxo and Ara-C as inhibitors of leukemic cells expressing the oncogene CBFB-MYH11, which causes AML. In vitro it was determined that Doxo and Ara-C affect the cell cycle of 3T3 cells, but have a lesser impact on ME-1 cells. In vivo WT mice can tolerate up to 10 doses of 6 mg/kg/every other day of Doxo, and 27 doses of 200 mg/kg/day of Ara-C. Leukemic mice treated with chemo drugs 4 weeks after transplantation survived 39 days with treatment, while mice treated 1 week after transplantation survived 19 days with treatment. Our research shows that two factors in decreasing toxicity and increasing efficacy of AML treatment are recovery time post irradiation and concentration of drug dose.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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