The Human Torque Teno virus (TTV) exhibits apoptotic activity through the expression of the VP3 protein. Homologous to TTV, the Chicken Anemia Virus (CAV) expresses Apoptin, which is also capable of inducing apoptosis. Interestingly, this induction only occurs in transformed cells. To achieve this specificity, Apoptin undergoes nucleocytoplasmic shuttling dictated by two localization signals: a Nuclear Export Signal (NES) and a Nuclear Localization Signal (NLS). Using the VP3 gene of TTV we have created two truncated mutants, which were then inserted into GFP plasmid and transfected into transformed H1299 cells to assess functionality of the localization domains. Additionally, the complete gene encoding the VP3 protein was transfected into primary cells to determine apoptotic selectivity.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry