Faculty Advisor

Heilman, Destin

Abstract

The Human Torque Teno Virus (TTV) is a small non-enveloped anellovirus with a single stranded circular DNA genome. One of the TTV homologs, Chicken Anemia Virus (CAV), produces a similar protein known as Apoptin, which has been shown to multimerize and induce apoptosis selectively in transformed cells. In order to further examine the role of multimerization, using molecular cloning, we created two VP3 containing T-vector constructs, cloning intermediates, which can be used in future studies of TTV VP3 specifically in the area of multimerization. Also, we have shown that TTV VP3 has the ability to induce apoptosis in transformed cells via a caspase 3/7 dependent pathway, similar to that of CAV Apoptin.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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