Faculty Advisor

Ryder, Elizabeth F.

Center

UMMC / University of Massachusetts Medical Center

Abstract

The immune system is integral to human survival, therefore the ability to understand how the system functions is key to advancing medicine. This research aimed to determine if THP-1 derived macrophages would be a comparable model system for human dendritic primary cells. Chromatin immunoprecipitation and LPS time course RNA-Seq experiments were performed to gather epigenetic and transcriptional data from both cell types. A comparative analysis of enhancer landscape and gene expression profiles in both cell types showed that there is a subset of genes in THP-1 derived macrophages that are comparable to the human dendritic primary cells.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Major

Bioinformatics and Computational Biology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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