Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

The IMPAS family of proteases is not well characterized, but some members may be associated with neurodegenerative diseases, such as Alzheimer’s disease. In this project, potential IMPAS substrates and pathways were investigated in vitro by transfecting plasmids encoding various IMPAS proteins and potential substrates into HEK293 cells and monitoring the cellular levels of predicted substrate proteins by Western blots. The results show that ODZ4, Syntaxin 5A, and truncated ST14 (delta ST14) likely do not serve as substrates. Protease hIMP1 was validated to cleave HCV core protein, as in a previous report. A putative connection between IMPAS and the autophagy pathway was revealed.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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