Faculty Advisor

Adams, David S.

Faculty Advisor

Dempski, Robert E.

Abstract

The long interspersed nuclear element-1 (L1) is a transposable element comprising roughly 17% of the human genome. This MQP investigated two zinc finger proteins (ZNF), 146 and 507, as potential L1-binding proteins. Bioinformatics analysis identified potential ZNF binding sites on L1 elements. Binding to 146 was verified biochemically using pull-down assays. The binding for the ZNFs are highly conserved across primate L1s, with 507 showing a specific period of loss. Fluorescence microscopy confirmed ZNF nuclear localization. Protein overexpression resulted in toxicity and possible signs of cell phase arrest. Protein knockdown resulted in morphology similar to senescence. Immunoprecipitation of flag-tagged 507 followed by mass spectroscopy identified PRMT5 as a 507-binding protein.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Interdisciplinary

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

Chemistry and Biochemistry

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