The main challenge associated with engineering avascular tissue such as cartilage is overcoming the physiological nutrient perfusion limit of 100 µm. Using a 3D printer, a PLA tissue scaffold with a diameter of 6.2 mm was fabricated. The scaffold was threaded with degradable collagen sutures and filled with a chondrogenic cell line suspended in a 4% alginate hydrogel. The scaffold system successfully supported cell proliferation and differentiation for 1 week, indicating efficient nutrient perfusion to the center of the scaffold.
Worcester Polytechnic Institute
Major Qualifying Project
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