Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Vidali, Luis

Abstract

The mechanism of chloroplast motility in response to light is not yet completely understood. We are testing the hypothesis that the motor proteins kinesin-4IIs and kinesin-7Is transport chloroplasts on microtubules in Physcomitrella patens (P.patens) cells. We are using RNA interference (RNAi) to test their function based on knockdown expression. RNAi constructs were designed and are being assembled to target both kinesin sub-families. Initial results show that Kinesin-7Is RNAi gives a chloroplast dispersion phenotype indicating that it might be involved in chloroplast motility. The effects of Kinesin-4IIs knockdown are still under investigation.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2014

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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