The Mello lab recently identified an interaction between prg-1 and pie-1, which are important in germline specification in the nematode C. elegans. This project aimed to confirm the interaction by RNAi injections and study double mutants to better understand the nature of the interaction. This project tested the hypothesis that PRG-1 interacts with PIE-1 by immunoprecipitation (IP), which preliminarily suggests they do not interact. Results also found that prg-1 does not regulate PIE-1 expression and proteins were identified that interacted specifically with the PRG-1 protein. Future experiments are needed to understand the role PRG-1 and PIE-1 play together in germline specification, which have greater implications in understanding epigenetics and stem cell self- renewal.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Josephs, Sadie Saludes

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042612-123008

Advisor

• Heilman, Destin

Year

• 2012

Sponsor

• UMMC

Date created

• 2012-04-26

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright