Faculty Advisor

Heilman, Destin

Center

University of Massachusetts Medical Center

Abstract

The Mello lab recently identified an interaction between prg-1 and pie-1, which are important in germline specification in the nematode C. elegans. This project aimed to confirm the interaction by RNAi injections and study double mutants to better understand the nature of the interaction. This project tested the hypothesis that PRG-1 interacts with PIE-1 by immunoprecipitation (IP), which preliminarily suggests they do not interact. Results also found that prg-1 does not regulate PIE-1 expression and proteins were identified that interacted specifically with the PRG-1 protein. Future experiments are needed to understand the role PRG-1 and PIE-1 play together in germline specification, which have greater implications in understanding epigenetics and stem cell self- renewal.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Project Center

University of Massachusetts Medical Center

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