University of Massachusetts Medical Center
The Mello lab recently identified an interaction between prg-1 and pie-1, which are important in germline specification in the nematode C. elegans. This project aimed to confirm the interaction by RNAi injections and study double mutants to better understand the nature of the interaction. This project tested the hypothesis that PRG-1 interacts with PIE-1 by immunoprecipitation (IP), which preliminarily suggests they do not interact. Results also found that prg-1 does not regulate PIE-1 expression and proteins were identified that interacted specifically with the PRG-1 protein. Future experiments are needed to understand the role PRG-1 and PIE-1 play together in germline specification, which have greater implications in understanding epigenetics and stem cell self- renewal.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry