Faculty Advisor

Weathers, Pamela

Abstract

The microalgae Ettlia oleoabundans produces up to 80% of its dry weight in triacylglycerols and is a prime candidate for biodiesel production. This study explores methods for transformation of E. oleoabundans by electroporation. Both the antibiotics hygromycin and spectinomycin were effective for transformant selection. First apparent successful production of E. oleoabundans protoplasts was achieved. Because this species produces nearly wall-less zoospores during its life cycle, synchronization was attempted using light/dark cycling and nitrate starvation to increase concentrations of zoospores to improve transformation.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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