Faculty Advisor

Politz, Samuel M

Abstract

The nematode Caenorhabditis elegans is a model organism for investigating innate immunity using microbial pathogens. Saccharomyces cerevisiae, commonly known as baker's yeast, was found to associate with C. elegans by colonizing the intestinal lumen and causing intestinal distension. We found that when grown on S. cerevisiae, mutants carrying mutations in mitogen-activated protein (MAP) kinase kinase genes mek-1 and mek-2 accumulated intestinal S. cerevisiae more rapidly than wild type. Intact S. cerevisiae cells expressing an RFP-tagged protein were visible in the intestinal lumen by fluorescent microscopy, and their accumulation correlated with intestinal distension.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Share

COinS