Student Work

Increasing the Efficiency of Monoclonal Antibody Production by Isolating Splenic B Cells in Advance of Hybridoma Fusion

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The process of producing monoclonal antibodies using the hybridoma technology has been an arduous task for many years. In this study, we aim to improve the efficiency of monoclonal antibody production by isolating mature splenic B cells in advance of fusion using magnetic activated cell sorting (MACS) to select for cells expressing CD138, a cell marker present in mature B cells. Pre-isolating a population of cells expressing CD138+, but lacking CD19 and B220, resulted in a reduced number of cells required for fusion.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-011110-141433
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Year
  • 2010
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Date created
  • 2010-01-11
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Major
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Last modified
  • 2023-09-20

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Permanent link to this page: https://digital.wpi.edu/show/xd07gv212