The production of monoclonal antibodies (mAbs) is fast becoming a staple of pharmaceutical development. Understanding how differing feeds and bioreactor setups affects the production of mAbs is important for improving yields. In the process of manufacturing mAb IPTG1, an anti- phosphotyrosine antibody produced at Millipore, this project monitored cell viability and secreted antibody yields under a variety of culture conditions, including different media feeds and different gas spargers.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Ouellette, Christina Elizabeth

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050114-163918

Advisor

• Adams, David S.

Year

• 2014

Date created

• 2014-05-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright