Faculty Advisor

Buckholt, Michael Allan

Abstract

The purpose if this project is to identify a strain of rhizobia bacteria that forms the best symbiotic relationship with Chipilin, a legume, in order to make Chipilin a more profitable crop in New England. The two strains of rhizobia bacteria tested were PNL0i-Brady and USDA 3384. 168 plants were grown from seeds and inoculated either with each of the two strains alone, a combination of the two strains or no rhizobia as a control. Immunoblots were performed for leghemoglobin which resides in plant nodules, resulting from the Chipilin-rhizobia symbiosis process to determine the presence and extent of active symbiosis.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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