Student Work

A Method for the Tubular Self-Assembly of Cells to Create Tissue Engineered Blood Vessels

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The purpose of the project was to develop a method to induce the self-assembly of cells into a tubular construct. To do this, we utilized the concept of centrifugal force. Cells were inserted into a non-adhesive hollow polycarbonate mandrel. The mandrel was spun for 15 minutes, forcing the cells to distribute themselves against the inner wall of the mandrel. After spinning, the mandrel was placed in a bioreactor and incubated for 3 days. During incubation, the cells aggregated and contracted off the mandrel, forming a tubular construct. This cellular tube was then removed from inside the mandrel using forceps. Histology was used to examine tissue structure and distribution of cells. The harvested tubes exhibited dense aggregates of cells indicative of a cohesive tissue.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Creator
Publisher
Identifier
  • E-project-042210-182526
Advisor
Year
  • 2010
Date created
  • 2010-04-22
Resource type
Major
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Last modified
  • 2021-02-02

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Permanent link to this page: https://digital.wpi.edu/show/fj236368g