Adams, David S.
Rita Bortell, PhD, UMASS Medical School
The purpose of this project was to correlate the time of onset of pancreatic a-cell apoptosis with the onset of diabetes in the BioBreeding Diabetes Prone (BB/DP) rat model to provide an initial determination of whether apoptosis is a primary or secondary event in the disease onset. Immunofluorescence microscopy was used to assay the appearance of Annexin-V apoptotic protein marker and the appearance of DNA fragmentation (via TUNEL assays). Due to time constraints, confirmation of beta-cell apoptosis was not possible in this study; future research, however, should conclusively prove the role of beta-cell apoptosis in diabetes onset.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology