Faculty Advisor

Rolle, Marsha W.


The purpose of this project was to design a perfusion bioreactor to nourish the lumen of a tissue tube comprised of rat smooth muscle cells in order to eliminate luminal cell necrosis. Once the bioreactor was deemed suitable for living tissue, a 19-day old tissue tube was mounted inside the bioreactor and the bioreactor ran for 3 days inside an incubator. Control tissue tubes were grown statically on silicone mandrels for 3 days. Tissue tubes preserved on the same day that the bioreactor was put in the incubator also served as control tissues to compare the size and morphology of tissue tube cultured in the bioreactor with tissue tubes harvested prior to incubation. The bioreactor was designed to provide an innovative way to mount tissue tubes to introduce luminal perfusion.


Worcester Polytechnic Institute

Date Accepted

April 2011


Biomedical Engineering

Project Type

Major Qualifying Project



Advisor Department

Biomedical Engineering

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