Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

The purpose of this project was to determine whether protein Mdm2, a p53 regulator previously shown to interact with MdmX, is required for the p53-independent role of MdmX in genome stabilization and suppression of cell transformation in vitro. Triple knock-out (TKO) cells lacking p53, Mdm2, and MdmX were transfected with an MdmX expression plasmid. Compared to control cells, TKO cells ectopically expressing MdmX show decreased cell proliferation, a longer cell cycle, increased chromosome numbers and bipolar mitotic spindles, and decreased foci formation. Thus, MdmX, even in the absence of Mdm2, plays a role in genome stability and proliferation. This is crucial to consider in regards to potential cancer treatments aimed to suppress Mdm2 and/or MdmX in order to reactivate p53.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Humanities and Arts

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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