Gaudette, Glenn R
The purpose of this project was to develop a method for isolating cardiomyocytes (CMC) from human mesenchymal stem cells (hMSCs) after co-culturing. This was addressed by developing a device that achieved a viable homogenous CMC population. This would allow the CMCs to be implanted into the patient, without the risk of stem cells differentiating into various lineages other than cardiac cells. Research has shown that CMCs proliferate when co-cultured with hMSCs. Fluid leakage testing, a cell viability assay, immunohistochemistry staining, cell counts, and observation of morphological characteristics through visual microscopy were conducted. Design development resulted in an efficient cell culture method to maintain cell population separation, and improved CMC health.
Worcester Polytechnic Institute
Major Qualifying Project