The purpose of this project was to develop a method for isolating cardiomyocytes (CMC) from human mesenchymal stem cells (hMSCs) after co-culturing. This was addressed by developing a device that achieved a viable homogenous CMC population. This would allow the CMCs to be implanted into the patient, without the risk of stem cells differentiating into various lineages other than cardiac cells. Research has shown that CMCs proliferate when co-cultured with hMSCs. Fluid leakage testing, a cell viability assay, immunohistochemistry staining, cell counts, and observation of morphological characteristics through visual microscopy were conducted. Design development resulted in an efficient cell culture method to maintain cell population separation, and improved CMC health.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Gunderson, Alexandra
• Rosales, Mardelle Adriana
• DeMartino, Angelica Marie
• Chou, Danice Yequay

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042810-183852

Advisor

• Gaudette, Glenn R.

Year

• 2010

Date created

• 2010-04-28

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright

Last modified

• 2021-01-06