The purpose of this project was to generate a mouse line containing a heart-specific deletion of genes encoding JNK-1 and -2, which may map to insulin-resistance, to create a model for studying the effects of type II diabetes on cardiomyocytes. The Cre/loxP recombination system was used in a C57BL/6 mouse, with Cre under the control of a MHC promoter to drive expression in cardiomyocytes. PCR analysis showed the creation of 3 Cre-positive mice for JNK1, and 3 for JNK2, providing mice which can be used to determine the potential role of JNK1 and JNK2 in insulin-resistance in heart cells.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Loucagos, Dimitrios A.
• Otuyelu, Adeola Oluwakemi

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042712-182216

Advisor

• Adams, David S.

Year

• 2012

Sponsor

• Dr. Jason Kim

Date created

• 2012-04-27

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright