Faculty Advisor

Adams, David S.

Center

UMMC / University of Massachusetts Medical Center

Abstract

The purpose of this project was to generate a mouse line containing a heart-specific deletion of genes encoding JNK-1 and -2, which may map to insulin-resistance, to create a model for studying the effects of type II diabetes on cardiomyocytes. The Cre/loxP recombination system was used in a C57BL/6 mouse, with Cre under the control of a MHC promoter to drive expression in cardiomyocytes. PCR analysis showed the creation of 3 Cre-positive mice for JNK1, and 3 for JNK2, providing mice which can be used to determine the potential role of JNK1 and JNK2 in insulin-resistance in heart cells.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2012

Major

Biology and Biotechnology

Major

Society, Technology and Policy

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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