The purposes of this MQP were to identify and characterize novel missense mutations in the Caenorhabditis elegans gene mig-10 which cause defects in neuronal migration, axonal growth, and excretory cell growth. Because of its fully penetrant mutant phenotype characteristic in the null mutant, the truncated excretory canal phenotype was used as a genetic model for developmental axonal growth. The mutants RY0920, RY0921 and RY0922 were isolated by using a simple screen. RY0920 strain was confirmed as new allele of mig-10 by complementation test, PCR, and restriction digestion. Novel missense alleles of mig-10 can help to define MIG-10 protein domains and residues important for neuronal migration function in C. elegans.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Duval, Jo-Ellen Jean
• Zacharia, An T.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-043009-122725

Advisor

• Ryder, Elizabeth F.
• Buckholt, Michael Allan

Year

• 2009

Date created

• 2009-04-30

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2021-02-02