Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Ryder, Elizabeth F


The purposes of this MQP were to identify and characterize novel missense mutations in the Caenorhabditis elegans gene mig-10 which cause defects in neuronal migration, axonal growth, and excretory cell growth. Because of its fully penetrant mutant phenotype characteristic in the null mutant, the truncated excretory canal phenotype was used as a genetic model for developmental axonal growth. The mutants RY0920, RY0921 and RY0922 were isolated by using a simple screen. RY0920 strain was confirmed as new allele of mig-10 by complementation test, PCR, and restriction digestion. Novel missense alleles of mig-10 can help to define MIG-10 protein domains and residues important for neuronal migration function in C. elegans.


Worcester Polytechnic Institute

Date Accepted

April 2009


Biology and Biotechnology

Project Type

Major Qualifying Project



Advisor Department

Biology and Biotechnology

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