Faculty Advisor

Adams, David S.

Center

UMMC / University of Massachusetts Medical Center

Abstract

The Sec 61 complex in eukaryotes is a passive pore in the endoplasmic reticulum (ER) membrane that permits a secretory protein to enter the ER membrane or lumen. To facilitate understanding of channel opening and closing, and especially to help determine the location of the plug domain relative to key cysteine residues, several yeast Sec 61 mutants were constructed by site-directed mutagenesis of cysteine residues at positions C121, C150, and C373. The mutants expressed Sec 61 and showed growth defect phenotypes.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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