Adams, David S.
University of Massachusetts Medical Center
The Sec 61 complex in eukaryotes is a passive pore in the endoplasmic reticulum (ER) membrane that permits a secretory protein to enter the ER membrane or lumen. To facilitate understanding of channel opening and closing, and especially to help determine the location of the plug domain relative to key cysteine residues, several yeast Sec 61 mutants were constructed by site-directed mutagenesis of cysteine residues at positions C121, C150, and C373. The mutants expressed Sec 61 and showed growth defect phenotypes.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology