Faculty Advisor

Arguello, Jose' M.

Abstract

The Sinorhizobium meliloti genome encodes five Cu+-ATPases that aid in detoxification and regulate copper homeostasis via the efflux of copper from the cytoplasm to the periplasmic space. Bioinformatic analysis concluded that additional cuproproteins found in S. meliloti genome might interact with Cu+-ATPases to aid in copper homeostasis. Atomic Absorption Spectroscopy (AAS) was used to determine the concentration of copper in subcellular fractionation of wild type and mutant strains. Results suggest that copper distribution in different subcellular fractions is linked to the dynamic of copper transporters and cuproproteomes.

Publisher

Worcester Polytechnic Institute

Date Accepted

October 2013

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Advisor Program

Chemistry and Biochemistry

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