Faculty Advisor

Adams, David S.

Center

UMMC / University of Massachusetts Medical Center

Abstract

The Smurf2 gene in mice encodes a protein that mediates ubiquination and degradation of a germinal center transcription factor responsible for B-cell proliferation. Knockouts of the Smurf2 gene have been shown in our lab to increase the formation of Diffuse Large B-Cell Lymphomas. The purpose of this project was to clone and sequence the VH4 intron locus in the DNA from the germinal centers of spleens from Smurf2-KO mice to determine whether they have an increased DNA mutation rate prior to tumor formation. The cloning of the DNA was unsuccessful, indicating a need to conduct further experiments.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2014

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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