The Smurf2 gene in mice encodes a protein that mediates ubiquination and degradation of a germinal center transcription factor responsible for B-cell proliferation. Knockouts of the Smurf2 gene have been shown in our lab to increase the formation of Diffuse Large B-Cell Lymphomas. The purpose of this project was to clone and sequence the VH4 intron locus in the DNA from the germinal centers of spleens from Smurf2-KO mice to determine whether they have an increased DNA mutation rate prior to tumor formation. The cloning of the DNA was unsuccessful, indicating a need to conduct further experiments.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Keaffer, Lee William

Contributors

• Zhang, Hong
• Trabucco, Sally
• Schrader, Carol

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050114-084305

Advisor

• Adams, David S.

Year

• 2014

Sponsor

• University of Massachusetts Medical School

Date created

• 2014-05-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright