Faculty Advisor

Adams, David S.

Abstract

The Tbx family of transcription factors control many early cell fate decisions in a developing embryo. Mutations in these genes can result in devastating physical abnormalities. In order to determine the function of Tbx genes in early post-implantation mammalian development, RT-PCR techniques were used to analyze the expression of six Tbx genes: Tbx1, Tbx3, Tbx4, Tbx5, Tbx19 and Tbx20 in key stages of development in the mouse embryo. We determine that Tbx1, Tbx4, and Tbx20 were shown to be expressed from LVET to Streak stages - the later stages of development examined. Tbx3 was expressed at all stages examined, and Tbx5 and Tbx19 were not detected at any of the stages examined. Further analysis will determine their role in early mouse development.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2008

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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