The Torque Teno Virus (TTV) is a non-enveloped virus that has a single stranded circular genome. TTV contains a third virus protein (VP3) that induces apoptosis in cancer cells. TTV VP3 is known to have two regions of interest, the Nuclear Localization Sequence (NLS) and the Nuclear Export Sequence (NES). The NLS in TTV VP3 is composed of two basic regions separated by a non-specific region. Without the NLS, the peptide is punctate and cytoplasmically localized. A truncation mutation of the NLS was achieved, separating the two basic regions. This revealed that the first construct tended to self-aggregate in the cytoplasm. The second region showed no localization. Both regions are hypothesized to be necessary for the functionality of the structure and the apoptotic ability of the protein.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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Chemistry and Biochemistry