The yeast one-hybrid (Y1H) system is an important and powerful assay for the detection of protein-DNA interactions. However, the system cannot assay for heterodimeric transcription factors (TFs), key regulators of spatiotemporal gene expression. A modified Y1H system was developed using novel plasmid constructs to coexpress Gal4 activation domain (AD)-fusion heterodimer pairs in existing and newly constructed bait reporter yeast strains. The clones and strategies developed provide a foundation to effectively screen dimerizing TFs for sequence specific interactions.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Renda, Brian A.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042910-110836

Advisor

• Duffy, Joseph B.

Year

• 2010

Sponsor

• Dr. Marian Walhout UMass Medical School

Date created

• 2010-04-29

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright