Faculty Advisor

Duffy, Joseph B.

Abstract

The yeast one-hybrid (Y1H) system is an important and powerful assay for the detection of protein-DNA interactions. However, the system cannot assay for heterodimeric transcription factors (TFs), key regulators of spatiotemporal gene expression. A modified Y1H system was developed using novel plasmid constructs to coexpress Gal4 activation domain (AD)-fusion heterodimer pairs in existing and newly constructed bait reporter yeast strains. The clones and strategies developed provide a foundation to effectively screen dimerizing TFs for sequence specific interactions.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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