There is a need for an environmental chamber in the cellular and tissue engineering fields that combines the characteristics of a cell culture incubator and a biosafety cabinet for long-term maintenance of viable cell populations for complex cellular printing applications and live cell imaging under sterile culture conditions. A novel air curtain technology was developed and its effectiveness was evaluated. The design was selected for its low cost. Its ability to maintain appropriate conditions and prevent permeability of CO2 forced through the air curtain from outside was experimentally quantified. Our results indicate that the air curtain was able to maintain CO2 levels and prevent permeation. The temperature and humidity levels dropped equally to the controls.
Worcester Polytechnic Institute
Major Qualifying Project
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