Student Work

Freeze Tolerance in the Moss Physcomitrella patens

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This major qualifying project involves the freezing and thawing of moss species pre-cultured with Abscisic Acid (ABA) to promote freezing tolerance. The goal of this project was to determine if the moss species Physcomitrella patens could be frozen and re-grown by reproducing published protocols and developing a growth assay to test for viability. Moss plants were grown under LED lights and pre- cultured with cryoprotectants. The moss was then placed into cryovials and preserved for two days in a -80 °C freezer. After one week, moss from the cryovials was thawed and placed back onto PpNH4 for the test of re-growth. After 11 days the moss that was grown on ABA agarose plates and pre-treated with Dimethyl Sulfoxide (DMSO) and Glucose regenerated to pre-freezing amounts.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-042513-104945
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Year
  • 2013
Date created
  • 2013-04-25
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