Freeze Tolerance in the Moss Physcomitrella patens
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open in viewerThis major qualifying project involves the freezing and thawing of moss species pre-cultured with Abscisic Acid (ABA) to promote freezing tolerance. The goal of this project was to determine if the moss species Physcomitrella patens could be frozen and re-grown by reproducing published protocols and developing a growth assay to test for viability. Moss plants were grown under LED lights and pre- cultured with cryoprotectants. The moss was then placed into cryovials and preserved for two days in a -80 °C freezer. After one week, moss from the cryovials was thawed and placed back onto PpNH4 for the test of re-growth. After 11 days the moss that was grown on ABA agarose plates and pre-treated with Dimethyl Sulfoxide (DMSO) and Glucose regenerated to pre-freezing amounts.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042513-104945
- Advisor
- Year
- 2013
- Date created
- 2013-04-25
- Resource type
- Major
- Rights statement
Relations
- In Collection:
Items
Items
Thumbnail | Title | Visibility | Embargo Release Date | Actions |
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Jonathan_Cline_Eric_Johnson_Freeze_Tolerance_in_the_Moss_Physcomitrella_patens.pdf | Public | Download |
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