Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Vidali, Luis


This major qualifying project involves the freezing and thawing of moss species pre-cultured with Abscisic Acid (ABA) to promote freezing tolerance. The goal of this project was to determine if the moss species Physcomitrella patens could be frozen and re-grown by reproducing published protocols and developing a growth assay to test for viability. Moss plants were grown under LED lights and pre- cultured with cryoprotectants. The moss was then placed into cryovials and preserved for two days in a -80 °C freezer. After one week, moss from the cryovials was thawed and placed back onto PpNH4 for the test of re-growth. After 11 days the moss that was grown on ABA agarose plates and pre-treated with Dimethyl Sulfoxide (DMSO) and Glucose regenerated to pre-freezing amounts.


Worcester Polytechnic Institute

Date Accepted

April 2013


Biology and Biotechnology

Project Type

Major Qualifying Project



Advisor Department

Biology and Biotechnology