Faculty Advisor

Adams, David S.

Center

Worcester, MA

Abstract

Because HIV and other lentiviruses can uniquely stably replicate within non-dividing cells, we hypothesized that lentiviruses must utilize a special mechanism allowing them to interact with host cell nuclear pore complexes, thus granting the HIV pre-integration complex (PIC) access to the nucleoplasm by a common nuclear import process. To prove this hypothesis, we performed RNAi-mediated knockdown screening of several nuclear pore complex proteins. This led to the identification of nucleoporin-85 (nup85) as indispensable for HIV replication in macrophages. To eliminate siRNA non-specific events as contributory to the observed inhibition, an siRNA resistant mutant nup85 was created in order to rescue replication.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2007

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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