Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

This MQP researched transcription factor YY1 in B-cells, which is thought to be a master regulator of germinal center (GC) formation. GCs within lymphoid tissues are important areas of B-cell proliferation and differentiation, and are essential for a B-cell immune response. This immune response was studied by creating YY1-null mice lacking this key transcription factor. Using a cre/lox recombination system, mice were bred to become GC YY1-null. Fluorescence activated cell sorting (FACS) was performed for GC proteins GL-7 and CD-95 to quantitate changes in GC numbers. The results showed that stimulation of the mouse immune system with sheep red blood cell injection increases GC numbers, and deletion of YY1 causes GC numbers to decrease, suggesting that YY1 is important for GC formation.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2015

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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