This project aimed to advance the search for some of the synergistic compounds present in the plant, namely flavonoids. To verify the identities of chrysosplenol-D, chrysoplenetin, and cirsilineol, various TLC conditions were compared and then used to analyze fresh plant extracts from both plant shoot tip extracts and mature leaves. To profile the many flavonoids and terpenes in this species the best TLC method used was the mobile phase 36:9:5, benzene-pyridine-formic acid with silica gel as the stationary phase. There were at least six flavonoids and six terpenes present in the leaf and tip extracts. These results will improve our understanding of the changes in these compounds as plants grow to maturity and will help further the work on using this plant for treatment of malaria.
Worcester Polytechnic Institute
Major Qualifying Project
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Biology and Biotechnology