Faculty Advisor

Adams, David S.

Abstract

Bio Breeding (BB) rats are a well known model for juvenile diabetes. Diabetic prone (DP) and resistant (DR) lines were created by in-breeding. DP rats spontaneously develop diabetes via pancreatic beta cell death, but the exact mechanism remains unknown. This project investigated cellular levels of several proteins thought to play a role in endoplasmic reticulum (ER) stress, which could lead to beta cell death. Levels were compared in pancreatic islets from DP and DR thapsigargin-stressed and non-stressed rats by western blot and real-time PCR assays. The results indicated that DP rats manifested higher levels of ER stress proteins.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2006

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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