This project developed a delivery device to place multiple molecular stimuli in a C. elegans culture, and designed an experiment to determine receptor-ligand interactions through the behavioral analysis of C. elegans. The team designed a PDMS delivery device and stimuli including IAA and glycerol were transferred from a 96 well plate device to an OmniTray using the device as a stamp. The experiment was recorded and analyzed through MATLAB. The output of the analysis included heat maps and behavioral plots. These were used in adjunction with one another to analyze the behavioral responses of C. elegans to the stimuli. In future applications, this can be used to rapidly identify responses to stimuli exhibited by C. elegans, leading to a complete understanding of receptor-ligand interactions.
Worcester Polytechnic Institute
Major Qualifying Project
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