This project focused on investigating the binding properties of a panel of rabbit monoclonal antibodies (RmAbs) elicited by a novel vaccine consisting of an HIV-1 gp120 AE consensus DNA prime protein boost vaccine regimen. Recombinant gp120 proteins from several isolates in clade AE were produced by transient transfection of 293F cells. The gp120 proteins were purified by lectin beads, then verified for expression using SDS-PAGE and Western blots before being used as coats for ELISA to examine the reactivity of RmAb to these expressed gp120 proteins. Amino acid sequences of these gp120 proteins were used to map the potential epitopes recognized by the RmAbs. The data show that the novel vaccine was successful in producing RmAbs against several clade AE HIV-1 gp120 proteins.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Colpas, Emily Claire

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042213-151515

Advisor

• Adams, David S.

Year

• 2013

Sponsor

• Lu, Shan
• University of Massachusetts Medical School

Date created

• 2013-04-22

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright