This project focused on the localization of small RNAs as well as certain proteins in mammalian cells grown in culture. Immunofluorescence and confocal microscopy were used to locate signal recognition particle (SRP) RNA and three associated protein subunits, and also telomerase RNA and two associated proteins. Several different cell types were used to study the molecules, as well as multiple techniques for locating these molecules including fluorescence in situ nucleic acid hybridization, immunostaining, and fluorescent protein gene transfection and expression.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Citrone, James

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042507-154131

Advisor

• Wobbe, Kristin K.

Year

• 2007

Date created

• 2007-04-25

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright

Last modified

• 2020-12-27