Faculty Advisor

Wobbe, Kristin K

Abstract

This project focused on the localization of small RNAs as well as certain proteins in mammalian cells grown in culture. Immunofluorescence and confocal microscopy were used to locate signal recognition particle (SRP) RNA and three associated protein subunits, and also telomerase RNA and two associated proteins. Several different cell types were used to study the molecules, as well as multiple techniques for locating these molecules including fluorescence in situ nucleic acid hybridization, immunostaining, and fluorescent protein gene transfection and expression.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2007

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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