This project focuses on methods of improving aspects of a bioindicator protocol developed for the detection of benzo[a]pyrene (B[a]P), a procarcinogen identified in a local waterway. A hexane based extraction segment of the protocol was analyzed, and its efficiency determined for a range of sediment types. The effect of exposure time on a range of sediment types to B[a]P-contaminated water was investigated. Extraction efficiencies ranged from 16.5-40.6%, with coarser sediment yielding higher recovery. Sediment exposed to B[a]P-contaminated water for 1 week showed decreased yields as compared to 24-hour exposure (8.0-11.9% vs. 32.2-37.1%). Hydrophobic exclusion from water and concurrent association with sediment particles and adsorption into surface grooves was theorized to explain results.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Letourneux, James R.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042909-193640

Advisor

• Whitefleet-Smith, JoAnn L.

Year

• 2009

Date created

• 2009-04-29

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright