Whitefleet-Smith, JoAnn L.
This project focuses on methods of improving aspects of a bioindicator protocol developed for the detection of benzo[a]pyrene (B[a]P), a procarcinogen identified in a local waterway. A hexane based extraction segment of the protocol was analyzed, and its efficiency determined for a range of sediment types. The effect of exposure time on a range of sediment types to B[a]P-contaminated water was investigated. Extraction efficiencies ranged from 16.5-40.6%, with coarser sediment yielding higher recovery. Sediment exposed to B[a]P-contaminated water for 1 week showed decreased yields as compared to 24-hour exposure (8.0-11.9% vs. 32.2-37.1%). Hydrophobic exclusion from water and concurrent association with sediment particles and adsorption into surface grooves was theorized to explain results.
Worcester Polytechnic Institute
Major Qualifying Project
All authors have granted to WPI a nonexclusive royalty-free license to distribute copies of the work, subject to other agreements. Copyright is held by the author or authors, with all rights reserved, unless otherwise noted.
Biology and Biotechnology