Brain tumors, such as glioblastoma multiforme, have extraordinarily low five-year survival rates. Currently, it is difficult to monitor in real-time the progression of a tumor and how it responds to therapy. Biosensors been used to measure key metabolites, such as lactate and glutamate, at shorter time intervals to detect abnormal blood levels. In this study, an enzymatic coating to detect lactate was developed in vitro. Chitosan was used to immobilize lactate enzyme. The chitosan with the enzyme was coated a glassy carbon electrode and tested at different concentrations of aqueous lactic acid as a proof of concept.
Worcester Polytechnic Institute
Major Qualifying Project