Faculty Advisor

Srinivasan, Jagan

Abstract

Caenorhabitis elegans uses a system of chemosensation to survive, making it suitable for studying mechanisms of behavior. Octopamine Succinyl Ascaroside #9 (osas#9) is released by starving larvae and causes individuals to perform avoidance behaviors. Starved Young Adult worms attenuate avoidance by detecting an E.coli metabolite. Avoidance assays were performed with metabolites L-Proline and Cyclo(Phe-Pro), and on C.elegans loss of function mutants to determine specificity attenuation. Neither L-Proline nor Cyclo(Phe-Pro) in single or synergistic treatments recreated attenuation seen using E.coli extract, and attenuation was observed to be dependent on the presence of serotonin and gpa-2/gpa-3 alpha-subunits, implicating the ADF neuron as part of the neuronal circuit for attenuation.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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