Faculty Advisor

Rulfs, Jill

Abstract

Calponin is an actin-binding protein found in a variety of cells that is well known for its inhibition of actin-activated myosin ATPase. We hypothesize that calponin inhibits cellular secretion by two mechanisms. One mechanism is to prevent the cleavage of actin in the cytoskeleton, a gel-like mesh that acts as a physical barrier to vesicles travelling to the membrane, by gelsolin and other actin-severing proteins that cut paths for vesicles to travel. The other mechanism is to assist in the bundling and cross-linking of actin, creating smaller pores in the mesh that are more difficult for the vesicles to move through. This project conducted necessary background research, designed study and future experiments, and amplified pET and pCMV-HA vectors that will be used in the experiments.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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