Student Work

TESTING A LABEL-RETAINING FUNCTIONAL ASSAY FOR DETECTING CANCER STEM CELLS

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Cancer stem cells (CSCs) are a newly hypothesized subpopulation of cancer cells that are resistant to chemotherapy and may explain many cases of cancer resurgence. Unfortunately, these cells are difficult to detect and isolate for analysis. As CSCs cycle more slowly than actively dividing cancer cells, CSCs should retain any label that binds to intracellular molecules longer. A HCT116 human colorectal cancer line was pulsed with CFSE, then chased for 7 days, after which it was treated with various chemotherapy agents. FACS analysis indicated that two of the chemotherapy treatments may have enriched for label retaining cells, though none of the markers tested seem to possess potential for use as an HCT116 CSC marker.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-042711-143800
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Year
  • 2011
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Date created
  • 2011-04-27
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Last modified
  • 2023-11-07

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