Page, Raymond L.
This project set out to develop a system to mechanically stimulate in vitro skeletal muscle tissue to produce more accurate models of in vivo tissue for use in studying human muscular diseases. Active and passive contractions play a key role in the in vivo development of skeletal muscle. A device was produced in which tissue was cultured in fibrin gel to grow dogbone-shaped tissue around sets of posts in the device. The device is able to: statically or cyclically strain the tissue, control the amount of strain from -50% to +50%, allow for stimulation of up to 96 samples, and minimize the construct size of the tissues. Mechanical stimulation by the device led to greater myofiber alignment, higher fiber density, and overall a closer resemblance to in vivo tissue.
Worcester Polytechnic Institute
Major Qualifying Project
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