Student Work

Generation of a Recombinant Vaccinia Virus Expressing the New H5 Subtype of the Influenza A Hemmagglutinin Gene

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This project was designed to generate a recombinant vaccinia virus expressing the new H5 subtype of the HA gene as part of eventually defining the CTL epitope on the avian HA protein recognized by this human T cell clone. The result was achieved by first generating a transfer vector for homologous recombination. The vector was used to generate a recombinant vaccinia virus expressing the HA gene. Expression of the HA gene product was confirmed at both transcriptional and translational levels, using RT-PCR and immunoprecipitations, respectively.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-042605-194234
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Year
  • 2005
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Date created
  • 2005-04-26
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Permanent link to this page: https://digital.wpi.edu/show/rn3013048