Faculty Advisor

Adams, David S.

Center

Worcester, MA

Abstract

This project was designed to generate a recombinant vaccinia virus expressing the new H5 subtype of the HA gene as part of eventually defining the CTL epitope on the avian HA protein recognized by this human T cell clone. The result was achieved by first generating a transfer vector for homologous recombination. The vector was used to generate a recombinant vaccinia virus expressing the HA gene. Expression of the HA gene product was confirmed at both transcriptional and translational levels, using RT-PCR and immunoprecipitations, respectively.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2005

Major

Biology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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